Introduction & Basic Concepts

I’ve recently realised it can take a lot of reading, searching, and other hard work to even begin to understand this subject. Whether it’s called probiotic / biointensive / no-till growing it can all be daunting, especially if it’s your medicine or livelihood on the line. So, in an effort to ease that, I’ve written this short introduction into the topic. If anything’s unclear, or you ever need one on one help just message me on facebook.

What does probiotic growing even mean, and is it the same as XXXX?

This is one of the most common questions I’m asked, so before I dig into the science, I’d like to address this real quick.

Despite the inclusion of new vocabulary to help inform people what grow methods are being employed, it’s ended up in a lot of misused terms, and confusion. This confusion is understandable, the terminology is pretty scattered. In an attempt to clear this up, I’ve been spending some time tracking down the origins, division of terms, and their interconnections and this is what I’ve found.

KNF (Korean Natural Farming) – KNF is an accumulation of farming techniques, that are widely accepted as required reading for Probiotic Farming practices, as many of the practices originated from there, such as BIM or FPE. I can’t track down the origination of KNF, but I can provide a link to one of the most informative KNF sites I’ve come across.

No Till Farming – This is the practice of planting your next plant into the soil, right as one is coming out, so as to not disturb the pre-equististing microbial / fungal network in the soil, at its most basic.  It has a number of proven benefits, like increasing carbon sequestration by the soil(1)(2), reducing fossil fuel usage in major agriculture dramatically (2), reducing soil erosion(3), and controlling soil moisture evaporation better than traditional farming practices(3). No Till can be done in beds, shared beds, or even the traditional potted plant set ups, however with pots make sure to by a little bigger pots (10 – 15 Gallons is a good minimum), utilize a mulch, and fabric / breathing pots are highly recommended.

Probiotic Farming – In the simplest form, it’s a focus on soil dwelling bacterial and fungal homeostasis in the garden. In practice, this usually means an amalgamation of KNF, traditional organic growing techniques (composting, aerated teas), and either ROLS or No Till depending on how the soil is reused, with a few unique practices, such as anaerobic teas thrown in. Understandably, this can be a bit daunting, but hopefully this site can serve as a bit of a companion in taking in all of these system and consolidating them to what’s needed for you. Why call it probiotic you may ask? Well, you are probably familiar with ‘probiotic‘ bacteria, usually in relation to cultured and/or fermented foods (kimchi, sauerkraut, yogurt). When we buy probiotic food, we are buying something with live cultures of beneficial bacteria.  Often, the bacteria that are helpful in our intestines, can catch a happy ride into us by being helpful to the soil and plants as well. With probiotic farming practices, we’re reintroducing beneficial bacteria and fungi into the soil, while using growing methods that promote the proliferation of these soil dwelling colonies, using amendments that help them thrive, and in return, the microlife does most of the hard work. Sadly, in more common agricultural practices these beneficial colonies are lacking, which has played a part in our ever rising rate of over farming of agricultural land(4).

Soil probiotics are commonly known as soil-based organisms (SBOs). SBOs are referred to a probiotics because they are beneficial bacteria that live in the soil. “Until the 19th century, when food processing replaced hand-to-mouth ingestion of raw fruit and vegetables, [SBOs] formed a regular part of our diet.” Soil based organisms are considered “friendly” non-resident or transient microorganisms. “Transient micro organisms are different from resident micro organisms in that they do not take up permanent residence in the gastrointestinal tract. Instead, they establish small colonies for brief periods of time before dying off or being flushed from the intestinal system via normal digestive processes, or by peristaltic bowel action.” Even though these types of beneficial bacteria are only in the digestive system on a temporary basis, “they contribute to the overall function and condition of the digestive system.” From

1 – Current Status of Adoption of No-till Farming in the World and Some of its Main Benefits

2 – Preliminary estimates of the potential for carbon mitigation in European soils through no-till farming

3 – Evolution of the plow over 10,000 years and the rationale for no-till farming

4 – Organic agriculture key to feeding the world sustainably

Common Acronym You Will Need To Know

Check out this wonderful PDF guide here! 

IMO – Indigenous Microorganism – Often used interchangeably with the acronym BIM, ( meaning Beneficial Indigenous Microorganism. )

BIM – Beneficial Indigenous Microorganisms – A culturing method for transplanting microbes from a thriving biointensive (lots of bacterial / fungal life) ecosystem to a new ecosystem. For more info click here

EWC – Earthworm castings / compost – For a wonderful write up on the difference in vermicompost vs castings read this. Once you’ve read that, you’re checked that out I want to convey how SERIOUSLY important quality EWC is. If your soil is a house, the EWC & your compost are the foundation. They are your most reliable home for the colony of biotic life you are bringing into your garden. I use Malibu Compost & EWC from BuildASoil, in addition to whatever compost I make at home.

SST – Sprouted Seed Tea – These provide hormones, amino acids, enzymes, and tons of other wonderful compounds. The most commonly used are barley (which I use mostly for amino acid content), corn (which I use for cytokinin content), and alfalfa (which I use mostly for triacontanol content). Great recipe on how to use, from Some Old Coot and can be found here.

Do keep in mind that triacontanol is so strong you’ve got to use 1/3 the amount of any other seed.
There’s a wealth of content on this but to get started check out this photo tutorial.

Modern Gardening Notes:
Use alfalfa SST when transitioning to flower to reduce internodal space.
Use barley SST once a week in veg, and at week four of flower in conjunction with corn SST.
Use Corn SST once a week for first three – four (up to week 7 in on a 12 week plant, up to week 9 in a 14 week plant) weeks of flower.
Every other SST in veg I use the 1/2 cup of flax with the barley.
Past two nodes, they start getting full strength SST.
At one node I give them 1/3 strength SST.
No SST for sprouts
The high enzymatic content of barley aids in the conversion of dead roots by beneficial bacteria, keeping roots healthy, and the soil moving (in terms of the soil food web). This and much more is accomplished, in part, because enzymes lower the energy potential required to complete metabolic action by microbes, fungi, and the plant itself. This is one of the ways we can speed up the actions happening in the soil-food web, while maintaining balance of the life in the soil. (a concept I like to call ‘Accelerated Homeostasis’; more on that another time)
Alfalfa’s tricanotol rate when used correctly will thicken root mass, and keep slightly shorter internodal space (esp. when used during transition to flower), by making the plant focus on lengthening / strengthening the branches which allows it to more easily support higher yields. Try using Corn at double strength (twice the amount sprouted), with the normal 1/3 a standard measurement for the alfalfa (don’t mess with high Alf Seeds, your plants will look like mutants if you do) – At this strength a plant really squats and become quite buff!
The corn SST provides the highest cytokinin, which promotes thicker stems, for better transport of nutrients, and of course the easier time supporting larger fruits.
Don’t sprout the flax, add it when blending / grinding the sprouted barley. The reason we don’t sprout it is that flax is mucilaginous it doesn’t sprout like most sprouts. This is because once it meets water it forms a gel sack which surrounds the seed. This evolved to interfere with bacterial bio-slime from what I’ve read. I use it almost solely for its unique fatty acid complex, so sprouting would cause it to start using these fatty acid to make hormones/phytohormones we are getting elsewhere.

SWC Seaweed Concentrate – Kelp, but treated with an acid to increase solubility. This akin to Orange Juice vs Tang, (Not sure who said that first, it was someone over at LOS Forum, cheers to them, it’s a perfect metaphor).

Ascophyllum nodosum extracts contain various betaines and betaine-like compounds. In plants, betaines serve as a compatible solute that alleviates osmotic stress induced by salinity and drought stress; however, other roles have also been suggested, such as enhancing leaf chlorophyll content of plants following their treatment with seaweed extracts. Yield enhancement effects due to improved chlorophyll content in leaves of various crop plants have been attributed to the betaines present in the seaweed. It has been indicated that betaine may work as a nitrogen source when provided in low concentration and serve as an osmolyte at higher concentrations Betaines have been shown to play a part in successful formation of somatic embryos from cotyledonary tissues and mature seeds of tea. – The use of seaweed-based products from Ecklonia maxima and Ascophyllum nodosum as control agents for Meloidogyne chitwoodi and M. hapla on tomato plants

Application of seaweeds and seaweed extracts triggers the growth of beneficial soil microbes and secretion of soil conditioning substances by these microbes. As mentioned, alginates affect soil properties and encourage growth of beneficial fungi. Ishii and others (2000) observed that alginate oligosaccharides, produced by enzymatic degradation of alginic acid mainly extracted from brown algae, significantly stimulated hyphal growth and elongation of arbuscular mycorrhizal (AM) fungi and triggered their infectivity on trifoliate orange seedlings. – Seaweed Extracts as Biostimulants of Plant Growth and Development

LAB – Lactobacillus Serum Click here for a guide to culturing LAB

EM-1 – Effective Microorganism. First defined by Teruo Higa in the paper: The Concept and Theories of Effective Microorganism

Effective Microorganisms, aka EM Technology, is a trademarked term now commonly used to describe a proprietary blend of 3 or more types of predominantly anaerobic organisms that was originally marketed as EM-1™ Microbial Inoculant but is now marketed by a plethora of companies under various names, each with their own proprietary blend. “EM™ Technology” uses a laboratory cultured mixture of microorganisms consisting mainly of lactic acid bacteria, purple bacteria, and yeast which co-exist for the benefit of whichever environment they are introduced, as has been claimed by the various em-like culture purveyors. It is reported to include:
Lactic acid bacteria: Lactobacillus plantarum; L. casei; Streptococcus Lactis.
Photosynthetic bacteria: Rhodopseudomonas palustris; Rhodobacter sphaeroides.
Yeast: Saccharomyces cerevisiae; Candida utilis (These species are no longer cultured for, but some some palustris does end up from the basic LAB process).
Actinomycetes (no longer used in the formulas): Streptomyces albus; S. griseus.

So these days, for one reason or another, what is sold as EM-1 is Lactobacillus plantarum, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus delbrueckii, Bacillus subtilis, Saccharomyces cerevisiae, Rhodopseudomonas palustris.

“Effective microorganisms make amino acids useful to plants, and organic acids, polysaccharides and vitamins strengthen their immune systems. EM1 consists of a water solution that contains compounds that promote nitrogen fixation and photosynthesis, along with lactic bacteria, yeast and other components that these microorganisms need to live ” – (Shablin, 2006)

Commonly, you will be referred here when you learn about EM-1. Which you will notice is the same recipe when we speak of LAB. This recipe gives you LAB, not EM-1, as there are no PNSB / Yeast.
What is sold here is different then when we make LAB. 

PNSB – Purple Non-Sulphur Bacteria – Rhodopseudomonas palustris, and other purple non-sulphur bacteria are powerhouse of bioremediation, among so many uses It’ll take me a while to source all the relevant studies on it. Some quick highlights from a grower’s perspective, keeps stagnant water from becoming a death trap for clones & beginners that are over watering, eats algae, helps keep balance of ph in the medium esp. in the first cycle or two of the soil.

FPE – Fermented Plant Extract – A great way to create a organic fertilizer for your garden. Wonderful introductions to this can be found here and another here.

AACT – Actively Aerated Compost Tea – Sometimes people just write ACT, still referencing the same thing; brewing tea with some form of oxygen stimulation (bubblers, whirlpool). You can find recipe on the Methods page.

SAR – Systemic Acquired Resistance – Please start by reading this journal . This is such a massive and important subject it will demand its own post.

IPM – Integrated Pest Management – This is one of our primary ways of increasing SAR, and utilizing practice that make our gardens inhospitable to pest. To take your first steps down this road read here.

Less common:
SA – Salicylic Acid

Biointensive Friendly / Probiotic Friendly Company

BuildASoil – Our go to amendment, tea, soil, and worm casting supplier. They vend Modern Mix (option under living organic soil), and Modern Microbes.
Gro-Kashi – Probiotic Bokashi containers Azomite, LAB, Plum Extract, Oat Bran, and Love.
Dragonfly Earth Medicine – A great source for biodynamic / probiotic teas & IPM brew!
Modern Microbes – My own blend of  nutrient  solubilizing bacteria, nitrogen fixing bacteria, mycos, various bacterial / fungal strains to promote vigor, accelerate fatty acid biosynthesis, as well as to outcompete botrytis, fusarium, and other pathogenic microorganism.

Further Reading

To truly embrace probiotic growing, you’ve got to embrace the autodidact within us all. So prepare to read, make notes, and experiment; And above all, SHARE! So, here’s some reading on a few different subjects to get you started.

Rock Dust
A Rock Dust Primer

Nitrogen Fixing Bacteria
N. Fixing Acetobacter
Acetobacter diazotrophicus sp. nov., a Nitrogen-Fixing Acetic Acid Bacterium Associated with Sugarcane

Acetobacter diazotrophicus, an indoleacetic acid producing bacterium isolated from sugarcane cultivars of Mexico

Coffea arabica L., a New Host Plant for Acetobacter diazotrophicus, and Isolation of Other Nitrogen-Fixing Acetobacteria

Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat

Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus

Isolation and characterization of a new extracellular polysaccharide from a cellulose-negative strain of Acetobacter xylinum

Production of indole-3-acetic acid and gibberellins A1 and A3 by Acetobacter diazotrophicus and Herbaspirillum seropedicae in chemically-defined culture media

N. FIxing Azospirillum 

Agro-process intensification: soil borne micro-bioreactors with nitrogen fixing bacterium Azospirillum brasilense as self-sustaining biofertiliser source for enhanced nitrogen uptake by plants

Enhanced Mineral Uptake by Zea mays and Sorghum bicolor Roots Inoculated with Azospirillum brasilense

Survival of Azospirillum brasilense in the Bulk Soil and Rhizosphere of 23 Soil Types


Enzyme / Hormones / Fatty Acids

Adenylate-forming enzymes

A New Type of Peroxisomal Acyl-Coenzyme A Synthetase from Arabidopsis thaliana Has the Catalytic Capacity to Activate Biosynthetic Precursors of Jasmonic Acid

Functional Compounds in Fermented Buckwheat Sprouts

Gaseous ethanol penetration of plant tissues positively affects the growth and commercial quality of miniature roses and dill

Relevance of microbial coculture fermentations in biotechnology

Milk Fat: Origin of Fatty Acids and Influence of Nutritional Factors Thereon



Biochar characteristics relate to its utility as an alternative soil inoculum carrier to peat and vermiculite

Biochar induced soil microbial community change: Implications for biogeochemical cycling of carbon, nitrogen and phosphorus

Impact of addition of biochar along with Bacillus sp. on growth and yield of French beans


EM1 & Bokashi

Effect of different concentrations of effective microorganisms (Baikal EM1) on the root collar diameter and height growth in the seedlings of Anatolian black pine

Effect of Heat-Sterilization and EM (Effective Microorganisms) Application on Wheat (Triticum aestivum L.) Grown in Organic-Amended Sandy Loam Soil

The effect of three organic pre-harvest treatments on Swiss chard (Beta vulgaris L. var. cycla L.) quality

Residual effect of Organic manure EM Bokashi applied to Proceeding Crop of Vegetable Cowpea (Vigna unguiculata) on succeeding Crop of Radish

Properties and Applications of an Organic Fertilizer Inoculated with Effective Microorganisms

Response of photosystem II and photosynthetic pigments to salt and Baikal EM1 in tree seedlings

Influence of EM Bokashi on Nodulation, Physiological Characters and Yield of Peanut in Nature Farming Fields

Growth and yield response of wheat to EM (effective microorganisms) and parthenium green manure

Field evaluation of effective microorganisms (EM ) application for growth, nodulation, and nutrition of mung bean

Evaluation of phenol degradation by effective microorganism (EM) technology with EM-1

Efficiency of Lime Sulfur in the Control of Two-Spotted Mite in Papaya in Conventional and Organic (Bokashi-EM) System

Effects of Organic Fertilizers and a Microbial Inoculant on Leaf Photosynthesis and Fruit Yield and Quality of Tomato Plants

Effect of treatment with probiotics as water additives on tilapia (Oreochromis niloticus) growth performance and immune response

The effect of prior incubation with glycyl-L-alanine on the uptake of peptides by Lactobacillus casei

Probiotics in Aquaculture – Benefits to the Health, Technological Applications and Safety


Isolation and growth of the phototrophic bacterium Rhodopseudomonas palustris strain B1 in sago-starch-processing wastewater

Finding a niche: The habits and habitats of purple non-sulfur bacteria

Photosynthetic electron transport and anaerobic metabolism in purple non-sulfur phototrophic bacteria

Growth characteristics of Rhodopseudomonas palustris cultured outdoors, in an underwater tubular photobioreactor, and investigation on photosynthetic efficiency


Cannabinoid / Terpene Biosynthesis

The hexanoyl-CoA precursor for cannabinoid biosynthesis is formed by an acyl-activating enzyme in Cannabis sativa trichomes

Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway

A chemotaxonomic analysis of Cannabinoid Variation in Cannabis

Antibacterial Cannabinoids from Cannabis sativa: A Structure-Activity Study

Biosynthesis of cannabinoids Incorporation experiments with 13C-labeled glucoses

Cannabichromene, a New Active Principle in Hashish

Cloning and over-expression of a cDNA encoding a polyketide synthase from Cannabis sativa

Identification of candidate genes affecting D9 -tetrahydrocannabinol biosynthesis in Cannabis sativa

The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms

The Inheritance of Chemical Phenotype in Cannabis sativa L.

The Gene Controlling Marijuana Psychoactivity

Tetrahydrocannabinolic Acid Synthase, the Enzyme Controlling Marijuana Psychoactivity, is Secreted into the Storage Cavity of the Glandular Trichomes

Prenylation of olivetolate by a hemp transferase yields cannabigerolic acid, the precursor of tetrahydrocannabinol

PKS Activities and Biosynthesis of Cannabinoids and Flavonoids in Cannabis sativa L. Plants


Microbiome / Rhizome

Endophytic fungi harbored in Cannabis sativa L.: diversity and potential as biocontrol agents against host plant-specific phytopathogens

Isolation of endophytic fungi from Cannabis sativa and study their antifungal potential

Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

Organic acids in the rhizosphere – a critical review

Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth:  a greenhouse trial

Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities

Introduction to Rhizobia

The effect of electrokinetics on soil microbial communities

Plant Rhizosphere Microbial Communities

Role of Phosphatase Enzymes in Soil

Microbial responses to salt-induced osmotic stress

Rhizosphere bacterial communities associated with disease suppressiveness stages of take-all decline in wheat monoculture

Applications of free living plant growth-promoting rhizobacteria

Plant root excretions in relation to the
rhizosphere effect

Release of Rhizobium spp. from Tropical Soils and Recovery for Immunofluorescence Enumeration



Arsenic removal from contaminated soil via biovolatilization by genetically engineered bacteria under laboratory conditions

Industrial hemp (Cannabis satia L.) growing on heavy metal contaminated soil: fibre quality and phytoremediation potential

Selective attachment processes in ancient gold ore beneficiation

Role of enzymes in the remediation of
polluted environments

Phytochelatins and Metallothioneins: Roles in Heavy Metal Detoxification and Homeostasis


Cation-Ion Exchange Capacity

A Review of the Use of the Basic Cation Saturation Ratio and the “Ideal” Soil

Influence of Calcium on the availability of other soil cations


Pest Control

Effects of growing containers containing diatomaceous earth on the population of fungus gnat

Experiments on population growth of the predatory nematode punctatus in laboratory culture with observations on life history

Combining Hexanoic Acid Plant Priming with Bacillus thuringiensis Insecticidal Activity against Colorado Potato Beetle

Synergistic antifungal activity of two chitin-binding proteins from spindle tree (Euonymus europaeus L.)


SAR & JAR (Salicylic Response and Jasmonic Response) 

Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana

The Role of Salicylic Acid and Jasmonic Acid in Pathogen Defence

Linking aboveground and belowground interactions via induced plant defenses


Tissue Culturing

Influence of cultivar, explant source and plant growth regulator on callus induction and plant regeneration of cannabis sativa l.

Karyological Studies in Root-Tip Cells of Cannabis sativa var. indica

Molecular analysis of genetic fidelity in Cannabis sativa L. plants grown from synthetic (encapsulated) seeds following in vitro storage

Plant Tissue Culture Techniques

A micropropagation system for cloning of hemp
(cannabis sativa l.) by shoot tip culture

Thidiazuron-induced high-frequency direct shoot organogenesis of Cannabis sativa L

The Biotechnology of Cannabis Sativa 2nd Edition